PhD, Associate Professor
We conduct mechanistic investigations on the metabolic changes induced by dietary, chemical, microbial, and pathophysiological challenges through a combination of mass spectrometry-based untargeted metabolomics and targeted metabolite analysis, stable isotope tracing, in vitro biochemical analysis, animal model, and human intervention treatment. Untargeted global metabolite profiling and targeted metabolite analysis in our lab cover both endogenous metabolites (lipids, amino acids, organic acids, aldehydes, ketones, and microbial metabolites) and exogenous metabolites (phytochemicals, pharmaceuticals, carcinogens, and their metabolites) in biofluids, wastes, tissue and cell extracts.
Google Scholar: http://scholar.google.com/citations?user=dTooB-kAAAAJ&hl=en
Jae Kyeom Kim; Daniel D. Gallaher; Chi Chen; Dan Yao; Sabrina P. Trudo. Apiaceous vegetable consumption decreases PhIP-induced DNA adducts and increases methylated PhIP metabolites in the urine metabolome in rats; Journal of Nutrition. 2015;145:442-451.
A R Weingarden; C Chen; A Bobr; D Yao; Y Lu; V Nelson; M J Sadowsky; A Khoruts; Microbiota Transplantation Restores Normal Fecal Bile Acid Composition in Recurrent Clostridium difficile Infection.; Am J Physiol Gastrointest Liver Physiol. . 2014;306:310-319.
R Song; C Chen; L J Johnston; B J Kerr; T E Weber; G C Shurson; Effects of feeding diets containing highly oxidized dried distillers grains with solubles and increasing vitamin E levels to wean-finish pigs on growth performance, carcass characteristics, and pork fat composition.; J Anim Sci. . 2014;92:198-210.
Ting Lei; Lei Wang; Chi Chen; Yinduo Ji; Metabolomic investigation of methicillin-resistant staphylococcus aureus; Methods in Molecular Biology. 2014;1085:251-258.
Alexa R. Weingarden; Chi Chen; Aleh Bobr; Dan Yao; Yuwei Lu; Valerie M. Nelson; Michael J. Sadowsky; Alexander Khoruts; Microbiota transplantation restores normal fecal bile acid composition in recurrent Clostridium difficile infection; American Journal of Physiology - Gastrointestinal and Liver Physiology. 2014;306(4):G310-G319.
Anamika Singh; Marvin L. Dirain; Andrzej Wilczynski; Chi Chen; Blake A. Gosnell; Allen S. Levine; Arthur S. Edison; Carrie Haskell-Luevano; Synthesis, biophysical, and pharmacological evaluation of the melanocortin agonist AST3-88: Modifications of peptide backbone at Trp 7 position lead to a potent, selective, and stable ligand of the melanocortin 4 receptor (MC4R); ACS Chemical Neuroscience. 2014;5(10):1020-1031.
Dan Yao; Xiaolei Shi; Lei Wang; Blake A. Gosnell; Chi Chen; Characterization of differential cocaine metabolism in mouse and rat through metabolomics-guided metabolite profiling; Drug Metabolism and Disposition. 2013;41(1):79-88
Fei Li; Xiaoyan Pang; Kristopher W. Krausz; Changtao Jiang; Chi Chen; John A. Cook; Murali C. Krishna; James B. Mitchell; Frank J. Gonzalez; Andrew D. Patterson; Stable isotope- and mass spectrometry-based metabolomics as tools in drug metabolism: A study expanding tempol pharmacology; Journal of Proteome Research. 2013;12(3):1369-1376.
Andrew D. Patterson; Bradley A. Carlson; Fei Li; Jessica A. Bonzo; Min-Hyuk Yoo; Kristopher W. Krausz; Marcus Conrad; Chi Chen; Frank J. Gonzalez; Dolph L. Hatfield; Disruption of thioredoxin reductase 1 protects mice from acute acetaminophen-induced hepatotoxicity through enhanced NRF2 activity; Chemical Research in Toxicology. 2013;26(7):1088-1096.
Tien-Yuan Wu; Tin Oo Khor; Jong Hun Lee; Ka Lung Cheung; Limin Shu; Chi Chen; Ah-Ng Kong; Pharmacogenetics, pharmacogenomics and epigenetics of Nrf2-regulated xenobioticmetabolizing enzymes and transporters by dietary phytochemical and cancer chemoprevention; Current Drug Metabolism. 2013;14(6):688-694.
Jie Cheng; Chi Chen; Krausz W. Kristopher; Soumen K. Manna; Mike Scerba; Fred K. Friedman; Hans Luecke; Jeffrey R. Idle; Frank J. Gonzalez; Identification of 2-piperidone as a biomarker of CYP2E1 activity through metabolomic phenotyping; Toxicological Sciences. 2013;135(1):37-47.
Chi Chen; Sangyub Kim; LC-MS-based metabolomics of xenobiotic-induced toxicities; Computational and Structural Biotechnology Journal. 2013;4(5).
Lei Wang; Chi Chen; Emerging applications of metabolomics in studying chemopreventive phytochemicals; AAPS Journal. 2013;15(4):941-950.
Y Lu; D Yao; C Chen; 2-Hydrazinoquinoline as a derivatization agent for LC-MS-based metabolomic investigation of diabetic ketoacidosis.; Metabolites. 2013;3:993-1010.
R Song; C Chen; L Wang; L J Johnston; B J Kerr; T E Weber; G C Shurson; High sulfur content in corn dried distillers grains with solubles (DDGS) protects against oxidized lipids in DDGS by increasing sulfur-containing antioxidants in nursery pigs.; J Anim Sci. . 2013;91:2715-2728.
Xiaolei Shi; Dan Yao; Chi Chen; Identification of N-acetyltaurine as a novel metabolite of ethanol through metabolomics-guided biochemical analysis; Journal of Biological Chemistry. 2012;287(9):6336-6349.
Xiaolei Shi; Dan Yao; Blake A. Gosnell; Chi Chen; Lipidomic profiling reveals protective function of fatty acid oxidation in cocaine-induced hepatotoxicity; Journal of Lipid Research. 2012;53(11):2318-2330.
Jung-Hwan Kim; Chi Chen; Ah-Ng Tony Kong; Resveratrol inhibits genistein-induced multi-drug resistance protein 2 (MRP2) expression in HepG2 cells; Archives of Biochemistry and Biophysics. 2011;512(2):160-166.
Joséphine Charve; Chi Chen; Adrian D. Hegeman; Gary A. Reineccius; Evaluation of instrumental methods for the untargeted analysis of chemical stimuli of orange juice flavour; Flavour and Fragrance Journal. 2011;26(6):429-440.
Mohamed A. Abdelmegeed; Kwan-Hoon Moon; Chi Chen; Frank J. Gonzalez; Byoung-Joon Song; Role of cytochrome P450 2E1 in protein nitration and ubiquitin-mediated degradation during acetaminophen toxicity; Biochemical Pharmacology. 2010;79(1):57-66
Limin Shu; Ka-Lung Cheung; Tin Oo Khor; Chi Chen; Ah-Ng Kong; Phytochemicals: Cancer chemoprevention and suppression of tumor onset and metastasis; Cancer and Metastasis Reviews. 2010;29(3):483-502
Jian-Hong Zhu; James P. McClung; Xiaomei Zhang; Manuel Aregullin; Chi Chen; Frank J. Gonzalez; Tae-Wan Kim; Xin Gen Lei; Comparative impacts of knockouts of two antioxidant enzymes on acetaminophen-induced hepatotoxicity in mice; Experimental Biology and Medicine. 2009;234(12):1477-1483.
Wenge Li; Nehal Thakor; Eugenia Y. Xu; Ying Huang; Chi Chen; Rong Yu; Martin Holcik; Ah-Ng Kong; An internal ribosomal entry site mediates redox-sensitive translation of Nrf2; Nucleic Acids Research. 2009;38(3):778-788:[gkp1048]
Chi Chen; Kristopher W. Krausz; Yatrik M. Shah; Jeffrey R. Idle; Frank J. Gonzalez; Serum metabolomics reveals irreversible inhibition of fatty acid β-oxidation through the suppression of PPARα activation as a contributing mechanism of acetaminophen-induced hepatotoxicity; Chemical Research in Toxicology. 2009;22(4):699-707.
Avantika Barve; Chi Chen; Vidya Hebbar; Joseph Desiderio; Constance Lay-Lay Saw; Ah-Ng Kong; Metabolism, oral bioavailability and pharmacokinetics of chemopreventive kaempferol in rats; Biopharmaceutics and Drug Disposition. 2009;30(7):356-365.
Chi Chen; Yatrik M. Shah; Keiichirou Morimura; Kristopher W. Krausz; Makoto Miyazaki; Terrilyn A. Richardson; Edward T. Morgan; James M. Ntambi; Jeffrey R. Idle; Frank J. Gonzalez; Metabolomics Reveals that Hepatic Stearoyl-CoA Desaturase 1 Downregulation Exacerbates Inflammation and Acute Colitis; Cell Metabolism. 2008;7(2):135-147.
Xiaochao Ma; Chi Chen; Kristopher W. Krausz; Jeffrey R. Idle; Frank J. Gonzalez; A metabolomic perspective of melatonin metabolism in the mouse; Endocrinology. 2008;149(4):1869-1879.
Chi Chen; Kristopher W. Krausz; Jeffrey R. Idle; Frank J. Gonzalez; Identification of novel toxicity-associated metabolites by metabolomics and mass isotopomer analysis of acetaminophen metabolism in wild-type and Cyp2e1-null mice; Journal of Biological Chemistry. 2008;283(8):4543-4559.
Nadine Dragin; Zhanquan Shi; Rajat Madan; Christopher L. Karp; Maureen A. Sartor; Chi Chen; Frank J. Gonzalez; Daniel W. Nebert; Phenotype of the Cyp1a1/1a2/1b1(-/-) triple-knockout mouse; Molecular Pharmacology. 2008;73(6):1844-1856.
Yatrik M. Shah; Shinji Ito; Keiichirou Morimura; Chi Chen; Sun-Hee Yim; Volker H. Haase; Frank J. Gonzalez; Hypoxia-Inducible Factor Augments Experimental Colitis Through an MIF-Dependent Inflammatory Signaling Cascade; Gastroenterology. 2008;134(7):2036-2048.e3.
Miroslav Dostalek; Klarissa D. Hardy; Ginger L. Milne; Jason D. Morrow; Chi Chen; Frank J. Gonzalez; Jun Gu; Xinxin Ding; Delinda A. Johnson; Jeffrey A. Johnson; et al.; Development of oxidative stress by cytochrome P450 induction in rodents is selective for barbiturates and related to loss of pyridine nucleotide-dependent protective systems; Journal of Biological Chemistry. 2008;283(25):17147-17157.
Reiko Kurotani; Takeshi Tomita; Qian Yang; Bradley A. Carlson; Chi Chen; Shioko Kimura; Role of secretoglobin 3A2 in lung development; American Journal of Respiratory and Critical Care Medicine. 2008;178(4):389-398.
Chi Chen; Xiaochao Ma; Michael A. Malfatti; Kristopher W. Krausz; Shioko Kimura; James S. Felton; Jeffrey R. Idle; Frank J. Gonzalez; A comprehensive investigation of 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP) metabolism in the mouse using a multivariate data analysis approach; Chemical Research in Toxicology. 2007;20(3):531-542.
Shinji Ito; Chi Chen; Junko Satoh; SunHee Yim; Frank J. Gonzalez; Dietary phytochemicals regulate whole-body CYP1A1 expression through an arylhydrocarbon receptor nuclear translocator-dependent system in gut; Journal of Clinical Investigation. 2007;117(7):1940-1950.
Yueying Zhen; Kristopher W. Krausz; Chi Chen; Jeffrey R. Idle; Frank J. Gonzalez; Metabolomic and genetic analysis of biomarkers for peroxisome proliferator-activated receptor α expression and activation; Molecular Endocrinology. 2007;21(9):2136-2151.
Chi Chen; Frank J. Gonzalez; Jeffrey R. Idle; LC-MS-based metabolomics in drug metabolism; Drug Metabolism Reviews. 2007;39(2-3):581-597.
Guoxiang Shen; Changjiang Xu; Chi Chen; Vidya Hebbar; Ah-Ng Tony Kong; p53-independent G1 cell cycle arrest of human colon carcinoma cells HT-29 by sulforaphane is associated with induction of p21CIP1 and inhibition of expression of cyclin D1.; Cancer chemotherapy and pharmacology. 2006;57(3):317-327.
Sarbani Giri; Jeffrey R. Idle; Chi Chen; T. Mark Zabriskie; Kristopher W. Krausz; Frank J. Gonzalez; A metabolomic approach to the metabolism of the areca nut alkaloids arecoline and arecaidine in the mouse; Chemical Research in Toxicology. 2006;19(6):818-827.
Chi Chen; Linghua Meng; Xiaochao Ma; Kristopher W. Krausz; Yves Pommier; Jeffrey R. Idle; Frank J. Gonzalez; Urinary metabolite profiling reveals CYP1A2-mediated metabolism of NSC686288 (aminoflavone); Journal of Pharmacology and Experimental Therapeutics. 2006;318(3):1330-1342.
Ling-Hua Meng; Uma Shankavaram; Chi Chen; Keli Agama; Hai-Qing Fu; Frank J. Gonzalez; John Weinstein; Yves Pommier; Activation of aminoflavone (NSC 686288) by a sulfotransferase is required for the antiproliferative effect of the drug and for induction of histone γ-H2AX; Cancer Research. 2006;66(19):9656-9664.
Yueying Zhen; Ondřej Slanař; Kristopher W. Krausz; Chi Chen; Josef Slavík; Kerry L. McPhail; T. Mark Zabriskie; František Perlík; Frank J. Gonzalez; Jeffrey R. Idle; 3,4-Dehydrodebrisoquine, a novel debrisoquine metabolite formed from 4-hydroxydebrisoquine that affects the CYP2D6 metabolic ratio; Drug Metabolism and Disposition. 2006;34(9):1563-1574
Avanthika Gopalakrishnan; Chang-Jiang Xu; Sujit S. Nair; Chi Chen; Vidya Hebbar; Ah-Ng Tony Kong; Modulation of activator protein-1 (AP-1) and MAPK pathway by flavonoids in human prostate cancer PC3 cells; Archives of Pharmacal Research. 2006;29(8):633-644.
Rong Hu; Tin Oo Khor; Guoxiang Shen; Woo-Sik Jeong; Vidya Hebbar; Chi Chen; Changjiang Xu; Bandaru Reddy; Kiran Chada; Ah-Ng Tony Kong; Cancer chemoprevention of intestinal polyposis in ApcMin/+ mice by sulforaphane, a natural product derived from cruciferous vegetable; Carcinogenesis. 2006;27(10):2038-2046.
Tin Oo Khor; Rong Hu; Guoxiang Shen; Woo-Sik Jeong; Vidya Hebbar; Chi Chen; Changjiang Xu; Sujit Nair; Bandaru Reddy; Kiran Chada; et al.; Pharmacogenomics of cancer chemopreventive isothiocyanate compound sulforaphane in the intestinal polyps of ApcMin/+ mice; Biopharmaceutics and Drug Disposition. 2006;27(9):407-420.
Vidya Hebbar; Guoxiang Shen; Rong Hu; Bok-Ryang Kim; Chi Chen; Peter J. Korytko; James A. Crowell; Barry S. Levine; A.-N. Tony Kong; Toxicogenomics of resveratrol in rat liver; Life Sciences. 2005;76(20):2299-2314.
Chi Chen; Tony Kong Ah-Ng; Dietary cancer-chemopreventive compounds: From signaling and gene expression to pharmacological effects; Trends in Pharmacological Sciences. 2005;26(6):318-326.
Woo-Sik Jeong; Young-Sam Keum; Chi Chen; Mohit R. Jain; Guoxiang Shen; Jung-Hwan Kim; Wenge Li; Ah-Ng Tony Kong; Differential expression and stability of endogenous nuclear factor E2-related factor 2 (Nrf2) by natural chemopreventive compounds in HepG2 human hepatoma cells; Journal of Biochemistry and Molecular Biology. 2005;38(2):167-176.
Changjiang Xu; Guoxiang Shen; Chi Chen; Céline Gélinas; Ah-Ng Tony Kong; Suppression of NF-κB and NF-κB-regulated gene expression by sulforaphane and PEITC through IκBα, IKK pathway in human prostate cancer PC-3 cells; Oncogene. 2005;24(28):4486-4495.
Wenge Li; Mohit R. Jain; Chi Chen; Xin Yue; Vidya Hebbar; Renping Zhou; A.-N. Tony Kong; Nrf2 possesses a redox-insensitive nuclear export signal overlapping with the leucine zipper motif; Journal of Biological Chemistry. 2005;280(31):28430-28438.
Chi Chen; A.-N. Tony Kong; Dietary chemopreventive compounds and ARE/EpRE signaling; Free Radical Biology and Medicine. 2004;36(12):1505-1516.
Rong Hu; Vidya Hebbar; Bok-Ryang Kim; Chi Chen; Bozena Winnik; Brian Buckley; Patricia Soteropoulos; Peter Tolias; Ronald P. Hart; A.-N. Tony Kong; In vivo pharmacokinetics and regulation of gene expression profiles by isothiocyanate sulforaphane in the rat; Journal of Pharmacology and Experimental Therapeutics. 2004;310(1):263-271.
Chi Chen; Daniel Pung; Vasco Leong; Vidya Hebbar; Guoxiang Shen; Sujit Nair; Wenge Li; A.-N. Tony Kong; Induction of detoxifying enzymes by garlic organosulfur compounds through transcription factor Nrf2: Effect of chemical structure and stress signals; Free Radical Biology and Medicine. 2004;37(10):1578-1590.
Zhi-Qiang Jiang; Chi Chen; Bo Yang; Vidya Hebbar; A.-N. Tony Kong; Differential responses from seven mammalian cell lines to the treatments of detoxifying enzyme inducers; Life Sciences. 2003;72(20):2243-2253.
Rong Hu; Bok Ryang Kim; Chi Chen; Vidya Hebbar; A.-N. Tony Kong; The roles of JNK and apoptotic signaling pathways in PEITC-mediated responses in human HT-29 colon adenocarcinoma cells; Carcinogenesis. 2003;24(8):1361-1367.
Chi Chen; Guoxiang Shen; Vidya Hebbar; Rong Hu; Edward D. Owuor; A.-N. Tony Kong; Epigallocatechin-3-gallate-induced stress signals in HT-29 human colon adenocarcinoma cells; Carcinogenesis. 2003;24(8):1369-1378.
Ah-Ng Tony Kong; Rong Yu; Vidya Hebbar; Chi Chen; Edward Owuor; Rong Hu; Rachel Ee; Sandhya Mandlekar; Signal transduction events elicited by cancer prevention compounds; Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2001;480-481:231-241.
A.-N. Tony Kong; Edward Owuor; Rong Yu; Vidya Hebbar; Chi Chen; Rong Hu; Sandhya Mandlekar; Induction of xenobiotic enzymes by the map kinase pathway and the antioxidant or electrophile response element (ARE/EpRE); Drug Metabolism Reviews. 2001;33(3-4):255-271.
Ah-Ng Tony Kong; Rong Yu; Chi Chen; Sandhya Mandlekar; Thomas Primiano; Signal Transduction Events Elicited by Natural Products: Role of MAPK and Caspase Pathways in Homeostatic Response and Induction of Apoptosis; Archives of Pharmacal Research. 2000;23(1):1-16.
Chi Chen; Rong Yu; Edward D. Owuor; A.-N. Tony Kong; Activation of Antioxidant-Response Element (ARE), Mitogen-Activated Protein Kinases (MAPKs) and Caspases by Major Green Tea Polyphenol Components during Cell Survival and Death; Archives of Pharmacal Research. 2000;23(6):605-612.
Rong Yu; Chi Chen; Yin-Yuan Mo; Vidya Hebbar; Edward D. Owuor; Tse-Hua Tan; A.-N. Tony Kong; Activation of mitogen-activated protein kinase pathways induces antioxidant response element-mediated gene expression via a Nrf2-dependent mechanism; Journal of Biological Chemistry. 2000;275(51):39907-39913.
Y Chen; M Lin; Y Xiao; W Gan; D Min; C Chen; Nutrition survey in dental fluorosis-afflicted areas.; Fluoride. 1997;30:77-80.
Y Chen; M Lin; Z He; Y Xiao; C Chen; D Min; Y Liu; M H Yu; Relationship between total fluoride intake and dental fluorosis in areas polluted by airborne fluoride.; Fluoride. 1996;29:7-12.
- 2008 NIH Fellows Award for Research Excellence
- 2007 ASPET Post Doctoral Scientist Award in Drug Metabolism (1st place)
- 2007 Keystone Symposia Scholarship in Bioactive Lipids
- 2002 AAPS Excellence in Graduate Research in Biotechnology